Catalogue of recombinant enzymes for proteomic research
| Class | Product Name | Action Sites | Application | Specification (lyophilized powder) |
| Sequencing Grade | Recombinant Lysine Endopeptidase (Lys-C) | Specifical cleaving at the carboxyl side of Lysine Residue including Pro-Lys, Asp-Lys and other sequences hard to be identified | ①Proteomic research ②Peptide mapping ③Protein sequencing ④Identification of phosphorylation ⑤Analysis of disulfide bond ⑥Analysis of N-glycan | 20μg |
| 20μg，5 vials | ||||
| Recombinant Tripsin (TP) | Specifical cleaving at the carboxyl side of Arg and Lys | ①Proteomic Research ②Peptide mapping ③Protein sequencing
| 20μg | |
| 20μg，5 vials | ||||
| RLys-C/RTP mix | Specifical cleaving at the carboxyl side of Arg and Lys | ①Peptide mapping, especially suitable for MS analysis with large molecular or high- hydrophobic proteins, with remarkable increase peptides number ②Quality control or analysis of MAb biologicals | 20μg | |
| 20μg，5 vials | ||||
| Endoproteinase Glu-C (V8) | Cleaving at the carboxyl side of Glu and Asp. Specific at Glutamyl bond at ammonium acetate（pH 4.0）or ammonium bicarbonate (pH 7.8) | ①Proteomic research ②Peptide mapping of insulin analogues ③Peptide mapping of MAb
| 50μg | |
| 50μg，5 vials | ||||
| Recombinant Carboxypeptidase B (CPB) | Preferential cleaving C-terminal basic amino acid including Lys, Arg, His
| ①C-terminal sequencing ②Quality control of Recombinant MAb ③Removing C-terminal His-tag | 20μg | |
| 20μg，5 vials | ||||
| Research and Analysis Use | Recombinant Enterokinase (EK) | Specifical cleaving at the carboxyl side of Asp-Asp-Asp-Asp-Lys | ①Removing fusion tag of recombinant protein ②Activating the enzymatic digestion of trypsinogen | 50μg |
| 50μg, 5 vials | ||||
| Recombinant Tobaccoetchvirus protease (TEV) | Specifical cleaving at the carboxyl side of Glu-Asn-Leu-Tyr-Phe-Gln-Gly | Removing fusion tag of recombinant protein | 50μg | |
| 50μg，5 vials |
Catalogue of recombinant enzymes for industrial manufacture
| Product | Character | Action Sites | Application | Specification (lyophilized powder) | Activity |
| Recombinant Lysine Endopeptidase (Lys-C) | Gene Source: L. enzymogenes Expression host: E.coli Tag-free | Cleaving at the carboxyl side of lysine residues | ①Enzymatic proinsulin digestion and transpeptidation of recombinant insulin or its analogues（e.g. Insulin aspart） ②Specific enzymatic digestion of recombinant polypeptide drugs | 10mg | ≥3AU/mg |
| 1g | |||||
| Recombinant Tripsin (TP) | Gene Source: Swine Expression host: P. pichia Tag-free | Cleaving at the carboxyl side of lysine or arginine residues | ① Enzymatic proinsulin digestion and transpeptidation of recombinant insulin ②Digestive treatment Padhereut cell cultures ③Tissue enzymatic extraction ④Hydrolysis of peptone preparation | 10mg | ≥3800USP/mg |
| 1g | |||||
| Recombinant Kexin (Kex2) | Gene Source: Yeast Expression host: P. pichia Tag-free | Cleaving at the carboxyl side of Arg-Arg or Lys-Arg | ① Specific enzymatic processing preparation of proinsulin glargine ② Enzymatic preparation of recombinant peptide by fusion expression | 10mg | ≥10U/mg |
| 1g | |||||
| Carboxypeptidase B (CPB) | Gene Source: mouse pancreas Expression host: P. pichia Tag-free | Removes C-terminal Lys, Arg, His | ① Removing C-terminal basic amino acid of protein drug (e.g. recombinant insulin or analogues) or others | 10mg | ≥150U/mg |
| 1g | |||||
| Recombinant Enterokinase (EK) | Gene Source: Swine Expression host: P. pichia Tag-free | Highly recognizing sequence ‘DDDDK’ and cleaving at the lysine (K) | Used as a processing enzyme in the fusion expressing polypeptide | 10mg | ≥10000U/mg |
| 1g | |||||
| Recombinant Tobaccoetchvirus protease (TEV) | Expression host: E. coli With His-tag | Recognizing the carboxyl side of sequence Glu-Asn-Leu- Tyr-Phe-Gln-(Gly/Ser) and cleaving between the Gln and Gly/Ser residues. | It can be used as a processing enzyme in the fusion expression of polypeptide drugs or others | 10mg | ≥1000U/mg |
| 1g | |||||
| Recombinant Methionine Adenosyltransferase (MAT) | Expression host: E. coli With His-tag | catalyzing the formation of S-adenosyl methionine by methionine and ATP | It can be used to produce S-adenosyl methionine | 10mg | ≥0.6U/mg |
| 1g | |||||
| Recombinant Arginase | Gene Source: Homo sapiens Expression host: E. coli Tag-free | Converting L-arginine into L-ornithine and urea | Used to produce natural ornithine | 10mg | ≥20U/mg |
| 1g | |||||
| Recombinant Arginine Deiminoenzyme | Gene Source: Mycoplasma hominis Expression host: E. coli Tag-free | Catalyzing the hydrolysis of L-arginine to produce citrulline | Used to produce natural citrulline | 10mg | ≥30U/mg
|
| 1g | |||||
| Recombinant 4-Hydroxyl Prolinase (P4H) | Gene Source: ascomycetes Expression host: E. coli Tag-free | Catalyzing L-proline into L-hydroxyproline | ①Used to produce L-hydroxyproline； ②Protine Hydroxylation of collagen or analogues | 10mg | ≥1.0U/mg |
| 1g | |||||
| Recombinant Human Hyaluronidase (PH20) | Gene Source: Homo sapiens Expression host: CHO Tag-free | Catalyzing the hydrolysis of large molecule hyaluronic acid | Produce low-molecular active hyaluronic acid (for cosmetics) | 0.1mg | ≥50, 000U/mg |
| 0.5mg |
We can provide customized package according to customers requirement
——Catalogue of recombinant enzymes for diagnosis——
| Product Name | Appearance | Source | Substrate-product | Application | Activity |
| Urease | White lyophilized powder | Pseudomonas | Urea—NH3 | Enzymatic determination of urea in clinical diagnosis
| ≥100U/mg |
| Sarcosine Oxidase | Yellow lyophilized powder | Bacillus sp. BSD-8 | Sarcosine—Glycine&Formaldehyde | Enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase and creatine amidinohydrolase | ≥8.0U/mg |
| Creatine Amidinohydrolase | White lyophilized powder | Pseudomonas putida | Creatine—Sarcosine | Enzymatic determination of creatinine when couple with creatine amidinohydrolase in clinical diagnosis | ≥40U/mg |
| Creatinine Amidohydrolase | White lyophilized powder | Pseudomonas | Creatinine—Creatine | Enzymatic determination of creatinine when coupled with creatine amidinohydrolase and sarcosine oxidase in clinical analysis | ≥150U/mg |
| Glycerol Dehydrogenase | White lyophilized powder | Escherichia coli CFT073 | Glycerol—Dihydroxyacetone |